BIO All cells have these two characteristics:
Carmine[ edit ] Carmine staining of a parasitic flatworm. Carmine is an intensely red dye used to stain glycogenwhile Carmine alum is a nuclear stain.
Carmine stains require the use of a mordant, usually aluminum. Coomassie blue[ edit ] Coomassie blue also brilliant blue nonspecifically stains proteins a strong blue colour. It is often used in gel electrophoresis.
Cresyl violet[ edit ] Cresyl violet stains the acidic components of the neuronal cytoplasm a violet colour, specifically nissl bodies. Often used in brain research.
Crystal violet[ edit ] Crystal violetwhen combined with a suitable mordant, stains cell walls purple. Crystal violet is the stain used in Gram staining. DAPI is also not visible with regular transmission microscopy.
It may be used in living or fixed cells. DAPI-stained cells are especially appropriate for cell counting. It also imparts a strong red colour to red blood cells.
Eosin may also be used as a counterstain in some variants of Gram staining, and in many other protocols. There are actually two very closely related compounds commonly referred to as eosin. Most often used is eosin Y also known as eosin Y ws or eosin yellowish ; it has a very slightly yellowish cast.
The other eosin compound is eosin B eosin bluish or imperial red ; it has a very faint bluish cast. The two dyes are interchangeable, and the use of one or the other is more a matter of preference and tradition.
Ethidium bromide[ edit ] Ethidium bromide intercalates and stains DNA, providing a fluorescent red-orange stain. Although it will not stain healthy cells, it can be used to identify cells that are in the final stages of apoptosis — such cells have much more permeable membranes.
Consequently, ethidium bromide is often used as a marker for apoptosis in cells populations and to locate bands of DNA in gel electrophoresis.
The stain may also be used in conjunction with acridine orange AO in viable cell counting. Acid fuchsine[ edit ] Acid fuchsine may be used to stain collagen, smooth muscle, or mitochondria.
Haematoxylin[ edit ] Haematoxylin hematoxylin in North America is a nuclear stain. Used with a mordant, haematoxylin stains nuclei blue-violet or brown. Hoechst stains[ edit ] Hoechst is a bis-benzimidazole derivative compound that binds to the minor groove of DNA. Often used in fluorescence microscopy for DNA staining, Hoechst stains appear yellow when dissolved in aqueous solutions and emit blue light under UV excitation.
There are two major types of Hoechst: Hoechst and Hoechst The two compounds are functionally similar, but with a little difference in structure. Hoechst contains a terminal hydroxyl group and is thus more soluble in aqueous solution, however this characteristics reduces its ability to penetrate the plasma membrane.
Hoechst contains an ethyl substitution on the terminal hydroxyl group i. Starch is a substance common to most plant cells and so a weak iodine solution will stain starch present in the cells. Iodine is one component in the staining technique known as Gram stainingused in microbiology. Malachite green[ edit ] Malachite green also known as diamond green B or victoria green B can be used as a blue-green counterstain to safranin in the Gimenez staining technique for bacteria.
It can also be used to directly stain spores. Methyl green[ edit ] Methyl green is used commonly with bright-field, as well as fluorescence microscopes  to dye the chromatin of cells so that they are more easily viewed.
Methylene blue[ edit ] Methylene blue is used to stain animal cells, such as human cheek cells, to make their nuclei more observable. Also used to stain the blood film and used in cytology.Sample potato osmosis lab report.
To receive the best grade in potato cells lab report,we recommend the below format which we have clearly explained it for you in a simple leslutinsduphoenix.com consult our experts for more detailed report per your instructions and academic level.
Plant cells are remarkable in that they have two organelles specialized for energy production: chloroplasts, which create energy via photosynthesis, and mitochondria, which generate energy through respiration, a particularly important process when light is unavailable. Staining is an auxiliary technique used in microscopy to enhance contrast in the microscopic image.
Stains and dyes are frequently used in biology and medicine to highlight structures in biological tissues for viewing, often with the aid of different leslutinsduphoenix.com may be used to define and examine bulk tissues (highlighting, for .
Diffusion and Cell Membranes – II Objectives 2. To determine if osmosis and diffusion both occur through a selectively permeable membrane.
3. To observe the effects of hypotonic, isotonic and hypertonic solutions on plant cells and animal 1% starch, % egg albumin, and 1% sodium chloride.
3. Tie the top of the tube with thread while. starch concentration lab. like the starch. Dialysis tubing is a semi-permeable membrane, like the plasma membrane of cells, that is used in a variety of experiments involving osmosis (Dialysis Tubing). This experiment resembled the natural process of osmosis that all living cells undergo as their semi-permeable plasma membrane allows water.
A few years back, I briefly covered a throwaway Yahoo! article about how “carbs will make you lose weight” because so many readers had emailed about it.
It turned out that the “carbs” in the article were resistant starch, a type of carbohydrate that our digestive enzymes cannot break down.